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Table 2 Amplification primers for NR3C1 gene exons

From: Sequencing the exons of human glucocorticoid receptor (NR3C1) gene in Han Chinese with high-altitude pulmonary edema

Exon Primer Sequence Tm (°C) Length (bp)
2-1 GR-EXON2-1F 5′-TCGGATCAGGAAGATAATGTGA-3′ 60 728
GR-EXON2-1R 5′-GATCTCCAAGGACTCTCATTCG-3′
2-2 GR-EXON2-2F 5′-AGACCAAAGCACCTTTGACATT-3′ 60 773
GR-EXON2-2R 5′-TTCCTACTTTCAAAAGGCCACT-3′
3 GR-EXON3-F 5′-GCACTTGAAGCCAGAGTTCAC-3′ 60 342
GR-EXON3-R 5′-CACCCTGAGAAATGAAAACCA-3′
4 GR-EXON4-F 5′-CAATACCTGTGGGTGTCTTGG-3′ 60 387
GR-EXON4-R 5′-TTCCCATTTTTATTGGGCAGT-3′
5 GR-EXON5-F 5′-TTGAATAAACTGTGTAGCGCAGA-3′ 60 501
GR-EXON5-R 5′-CACCTGTATTCACCTGACTCTCC-3′
6 GR-EXON6-F 5′-GACAGGGCTAATTGATCTCATTG-3′ 60 307
GR-EXON6-R 5′-ATCAGGAAAACATCAGCTGGTTA-3′
7 GR-EXON7-F 5′-TTGCAAAACAAAACAAAAATGTG-3′ 60 384
GR-EXON7-R 5′-GGTGTCACTTACTGTGCCTTTCT-3′
8 GR-EXON8-F 5′-GGATGACACAGTGAGACCCTATC-3′ 60 391
GR-EXON8-R 5′-TTGAACTCAAGCTATCACCAACA-3′
GR-EXON9α-F 5′-GGGAATTCCAGTGAGATTGGT-3′ 60 459
GR-EXON9α-R 5′-AACTGCTTCTGTTGCCAAGTC-3′
GR-EXON9β-F 5′-GTGTAACCCGGCTGGATAAAT-3′ 60 508
GR-EXON9β-R 5′-TCTGCTTTCAAACAGCACCA-3′
  1. Tm melting temperature