Table 2 Amplification primers for NR3C1 gene exons
Exon | Primer | Sequence | Tm (°C) | Length (bp) |
|---|---|---|---|---|
2-1 | GR-EXON2-1F | 5′-TCGGATCAGGAAGATAATGTGA-3′ | 60 | 728 |
GR-EXON2-1R | 5′-GATCTCCAAGGACTCTCATTCG-3′ | |||
2-2 | GR-EXON2-2F | 5′-AGACCAAAGCACCTTTGACATT-3′ | 60 | 773 |
GR-EXON2-2R | 5′-TTCCTACTTTCAAAAGGCCACT-3′ | |||
3 | GR-EXON3-F | 5′-GCACTTGAAGCCAGAGTTCAC-3′ | 60 | 342 |
GR-EXON3-R | 5′-CACCCTGAGAAATGAAAACCA-3′ | |||
4 | GR-EXON4-F | 5′-CAATACCTGTGGGTGTCTTGG-3′ | 60 | 387 |
GR-EXON4-R | 5′-TTCCCATTTTTATTGGGCAGT-3′ | |||
5 | GR-EXON5-F | 5′-TTGAATAAACTGTGTAGCGCAGA-3′ | 60 | 501 |
GR-EXON5-R | 5′-CACCTGTATTCACCTGACTCTCC-3′ | |||
6 | GR-EXON6-F | 5′-GACAGGGCTAATTGATCTCATTG-3′ | 60 | 307 |
GR-EXON6-R | 5′-ATCAGGAAAACATCAGCTGGTTA-3′ | |||
7 | GR-EXON7-F | 5′-TTGCAAAACAAAACAAAAATGTG-3′ | 60 | 384 |
GR-EXON7-R | 5′-GGTGTCACTTACTGTGCCTTTCT-3′ | |||
8 | GR-EXON8-F | 5′-GGATGACACAGTGAGACCCTATC-3′ | 60 | 391 |
GR-EXON8-R | 5′-TTGAACTCAAGCTATCACCAACA-3′ | |||
9α | GR-EXON9α-F | 5′-GGGAATTCCAGTGAGATTGGT-3′ | 60 | 459 |
GR-EXON9α-R | 5′-AACTGCTTCTGTTGCCAAGTC-3′ | |||
9β | GR-EXON9β-F | 5′-GTGTAACCCGGCTGGATAAAT-3′ | 60 | 508 |
GR-EXON9β-R | 5′-TCTGCTTTCAAACAGCACCA-3′ |