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Table 1 Sensitivity and specificity of the in-house TPO-Ab ELISA for 182 matched plasma, venous DBS (vDBS), and fingerprick DBS (fDBS) samples

From: Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots

 

True positives (TP)

True negatives (TN)

False negatives (FN)

False positives (FP)

Sensitivity (%)

Specificity (%)

TP/(TP + FN)

95% CI

TN/(TN+ FP) (%)

95% CI

Plasma

12

169

0

1

100

73.5–100%

99.4

96.8–100%

vDBS

12

168

0

2

100

73.5–100%

98.8

95.8–99.9%

fDBS

11

167

1

3

91.7

61.3–99.8%

98.2

94.9–99.6%

  1. Note that a cut-off of 26 IU/mL was used