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Table 3 Calculation of inter-assay reliability of the in-house ELISA based on three DBS controls

From: Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots

Plate no.

High DBS control [IU/mL]

Intermediate DBS control [IU/mL]

Low DBS control [IU/mL]

1

62.9

27.7

8.8

2

62.6

24.0

13.6

3

57.1

22.1

11.2

4

53.2

21.8

9.6

5

53.0

23.0

10.1

6

56.9

19.5

6.9

7

42.9

18.3

6.7

8

53.1

18.5

8.2

Mean

55.2

21.9

9.4

Stdev

6.4

3.1

2.3

CV

11.5

14.3

24.4