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Table 3 Calculation of inter-assay reliability of the in-house ELISA based on three DBS controls

From: Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots

Plate no. High DBS control [IU/mL] Intermediate DBS control [IU/mL] Low DBS control [IU/mL]
1 62.9 27.7 8.8
2 62.6 24.0 13.6
3 57.1 22.1 11.2
4 53.2 21.8 9.6
5 53.0 23.0 10.1
6 56.9 19.5 6.9
7 42.9 18.3 6.7
8 53.1 18.5 8.2
Mean 55.2 21.9 9.4
Stdev 6.4 3.1 2.3
CV 11.5 14.3 24.4