Physiological and psychological responses of young males during spring-time walks in urban parks

Background It is widely believed that contact with the natural environment can improve physical and mental health. Urban green spaces may provide city residents with these benefits; however, there is a lack of empirical field research on the health benefits of urban parks. Methods This field experiment was performed in May. Seventeen males aged 21.2 ± 1.7 years (mean ± standard deviation) were instructed to walk predetermined 15-minute courses in an urban park and a nearby city area (control). Heart rate and heart rate variability (HRV) were measured to assess physiological responses. The semantic differential (SD) method, Profile of Mood States (POMS), and State-Trait Anxiety Inventory (STAI) were used to measure psychological responses. Results Heart rate was significantly lower while walking in the urban park than while walking in the city street. Furthermore, the urban park walk led to higher parasympathetic nervous activity and lower sympathetic nervous activity compared with the walk through the city street. Subjective evaluations were generally in accordance with physiological reactions, and significantly higher scores were observed for the ‘comfortable’, ‘natural’, and ‘relaxed’ parameters following the urban park walk. After the urban park walk, the score for the ‘vigor’ subscale of the POMS was significantly higher, whereas that for negative feelings such as ‘tension-anxiety’ and ‘fatigue’ was significantly lower. The score for the anxiety dimension of the STAI was also significantly lower after the urban park walk. Conclusions Physiological and psychological results from this field experiment provide evidence for the physiological and psychological benefits of urban green spaces. A brief spring-time walk in an urban park shifted sympathetic/parasympathetic balance and improved mood state.

In our modern urbanized society, however, opportunities for such interactions with nature are limited. Of late, the potential health benefits of urban green spaces have been studied. Urban green space can enhance the city environment by influencing temperature, wind, humidity, rainfall, soil erosion, flooding, air quality, scenic quality, and plant and animal diversity [21]. In addition, urban green space may provide important social and psychological benefits that enrich human life [22]. Recent demographic studies have found a positive association between exposure to urban green space and the perceived general health of residents [23,24]. Living in areas with walkable green spaces also increased the longevity of senior citizens, independent of age, sex, marital status, baseline functional status, and socioeconomic status [25].
Most individuals in industrialized countries live in urban areas and will continue to do so for the foreseeable future [26]; therefore, any beneficial effects of urban green space can improve general health and longevity. However, there is a lack of empirical research on the therapeutic benefits of urban green space [27]. Many investigators have emphasized the importance of field research [6,10] that can examine the effects of real environments.
We have conducted such a field experiment during winter [28], but there have been no such studies conducted during spring. Therefore, the aim of this study was to examine the physiological and psychological responses of young males during spring-time walks in urban parks.

Methods
This field experiment was conducted in May 2013 in Kashiwanoha Park (hereafter referred to as the urban park) in Kashiwa City, Chiba Prefecture, Japan. A city area around the urban park (hereafter referred to as the city area) was selected as a control site.
Seventeen Japanese male university students aged 21.2 ± 1.7 years (mean ± standard deviation) participated in this experiment. Before the experiment, the subjects were fully informed about the aims and procedures involved. After receiving a description of the experiment, the subjects signed an agreement to participate. On the experimental day, alcohol and tobacco were prohibited. This study was performed according to the regulations of the Ethics Committee of the Center for Environment, Health and Field Sciences, Chiba University, Japan.
The subjects were instructed to walk predetermined courses through the urban park and the city area. Each course took about 15 minutes to complete. Subjects walked both courses on the same day (at 10 to 12 am and 1 to 3 pm), and the site order was counterbalanced across subjects. On the day of the experiment, the weather was sunny. A 15-minute walk was taken under direct sunlight in the city area at a temperature of 27.0 ± 1.7°C (mean ± standard deviation) and relative humidity of 37.3 ± 8.4% (mean ± standard deviation). In the urban park, the walk was taken under both direct sunlight and shade at a temperature of 24.7 ± 1.6°C and relative humidity of 39.2 ± 5.3%. The trees in the park had light green leaves, and the azaleas were in full bloom ( Figure 1). There were no significant differences in baseline physiological indices or average walking speed between the two environments. After walking, the subjects returned to a waiting room and completed several questionnaires. They rested for approximately 20 minutes, after which they walked the other experimental course.
Heart rate and heart rate variability (HRV) were measured to assess cardiovascular and autonomic nervous system responses. The HRV was measured using a portable electrocardiograph (Activtracer AC-301A, GMS, Tokyo, Japan), and frequency spectra were generated using an HRV software tool (MemCalc/Win, GMS, Tokyo, Japan). For real-time HRV analysis using the maximum entropy method, interbeat (R-R) intervals were obtained continuously. In this study, two broad HRV spectral components were calculated: low frequency (LF; 0.04 to 0.15 Hz) and high frequency (HF; 0.15 to 0.40 Hz). The HF component is an estimate of parasympathetic nervous activity, while the LF/HF ratio is an estimate of sympathetic nervous activity [29,30]. To normalize HRV parameters across subjects, we used natural logarithmic transformed values for the analysis [31]. The heart rate and HRV data were collected at 1-minute intervals at each experimental location, and the 15-minute average was compared between sites.
Three different questionnaires were used to investigate psychological responses after walking at each experimental site. The semantic differential (SD) method [32] used three pairs of adjectives on seven scales, including 'comfortable to uncomfortable', 'natural to artificial', and 'relaxed to awakening'. The Profile of Mood State (POMS) scores were determined for the following six subscales: 'tensionanxiety', 'depression', 'anger-hostility', 'fatigue', 'confusion', and 'vigor'. A short form of the POMS with 30 questions was used to decrease the burden on the subjects [33][34][35]. The State-Trait Anxiety Inventory (STAI) [36] was used to evaluate anxiety.
A paired t-test was used to compare the mean physiological parameters between the two walking sites. The Wilcoxon signed-rank test was used to analyze differences in psychological indices reported after walking in the two environments. All statistical analyses were performed using SPSS 20.0 (SPSS Inc, Chicago, IL, USA).
For all cases, P < 0.05 (one sided) was considered statistically significant.

Results
The subjects exhibited significant differences in physiological responses during a fifteen-minute walk in two distinct environments, an urban park and a nearby city area. The mean baseline heart rate did not differ significantly between sites before the walk (urban park: 87.5 ± 3.1 bpm (mean ± standard error), city area: 86.1 ± 2.9 bpm; P > 0.05); however, all mean heart rate values within oneminute epochs were lower during the urban park walk compared with those during the city walk ( Figure 2A). The mean heart rate over the entire 15-minute period was significantly lower (by 4.0%) during the urban park walk than during the city walk (urban park: 86.7 ± 2.9 bpm, city area: 90.3 ± 2.6 bpm; P < 0.05; Figure 2B).
In addition, there were significant differences in HRV between the two sites. The mean normalized highfrequency (HF) component (ln(HF)), an estimate of parasympathetic nervous activity, was not significantly different at the start of the walk (urban park: 4.0 ± 0.2 msec 2 , city area: 4.3 ± 0.3 msec 2 ; P > 0.05), while most mean ln (HF) values within one-minute epochs were higher during the urban park walk than during the city walk ( Figure 3A). For the entire 15-minute duration, ln(HF) was 17.1% higher during the urban park walk than during the city area walk (urban park: 4.1 ± 0.2 msec 2 , city area: 3.5 ± 0.2 msec 2 ; P < 0.01; Figure 3B). In contrast, the natural logarithm of LF/HF (ln(LF/HF)), an estimate of sympathetic nervous activity, was lower for most one-minute epochs during the urban park walk ( Figure 4A), while the average ln(LF/HF) over 15 minutes was 20.3% lower than that for the city walk (urban park: 1.65 ± 0.18, city area: The urban park The city area  2.07 ± 0.18, P < 0.01; Figure 4B). Again, there was no significant difference in baseline ln(LF/HF) values between sites (urban park: 2.14 ± 0.23, city area: 1.67 ± 0.35; P > 0.05). Analysis of responses to three questionnaires completed after the urban park and city area walks, the SD method, the POMS scores, and the STAI scores revealed differences in psychological responses between the two environments. Significantly higher SD scores were observed following the urban park walk compared with those following the city walk for the following three adjectives: 'comfortable', 'natural', and 'relaxed' (P < 0.05; Figure 5). Differences were also detected in the POMS test ( Figure 6), with scores for the negative subscales of 'tension-anxiety' and 'fatigue' being significantly lower after walking in the urban park than after walking in the city area (P < 0.05). Conversely, the positive mood state 'vigor' was significantly higher after the urban park walk (P < 0.05). There were no significant differences in the scores for 'depression', 'anger-hostility', and 'confusion'. Finally, the total STAI score was 14.3% lower after the urban park walk compared with that after the city area walk (urban park: 41.6. ± 7.0 (mean ± standard deviation), city area: 48.6 ± 6.3; P < 0.05; Figure 7).

Discussion
Access to urban green spaces may have significant physiological and psychological effects on urban residents.
Compared with those after a brief walk in the city area, the heart rate was significantly lower (−4.0%), parasympathetic nervous activity was enhanced (17.1%), and sympathetic nervous activity was suppressed (−20.3%) during a brief walk in the urban park. These results are consistent with those from previous studies on physiological responses to forest settings [9,[11][12][13][14][15], suggesting that even small natural areas within a larger urban area can confer similar health benefits. These same HRV responses are often detected during massage [37,38] or yoga [39], so walking in an urban park may be a simple, accessible, and cost-effective method to improve general cardiovascular and mental health.
According to the analysis of the three questionnaires, the subjects in this study felt more 'comfortable', 'natural', 'relaxed', and 'vigorous' after a walk in the urban park. In addition, negative emotions and anxiety were significantly lower after the urban park walk. These results on the psychological benefits of walking in an urban park are partly consistent with previous findings [5,[9][10][11][12]15]. Because mental health is considered to be important in modern times [40], the psychological benefits of urban green space are expected to play a very important role in the promotion of mental health.
In a previous study, we examined the effects of urban green space in winter using the same experimental design and locations [28]. The current results corroborate our previous findings with one notable difference. In winter, a significant difference in sympathetic nervous activity was not detected between sites, while all other parameters (parasympathetic nervous activity, heart rate, and psychological indices) showed similar differences between walking environments.
Although we are now living in largely artificial urban environments, our physiological functions evolved in the natural environment [3,20,41]. The human tendency to seek natural environments implies that contact with nature may be important for promoting human health and well being [42]. The beneficial effects of urban green space suggest a simple accessible pathway to improved ** *  Figure 5 Comparison of subjective scoring for 'comfortable', 'natural', and 'relaxed' feelings between the two environments according to the semantic differential (SD) method. N = 17, mean ± standard deviation. *P < 0.05, **P < 0.01, determined by the Wilcoxon signed-rank test.  health. Furthermore, urban planners should pay more attention to maintaining and increasing accessible greenery in urban areas to improve the quality of life of the residents.
These findings provide empirical evidence for the physiological and psychological benefits of brief walks in an urban park. However, these results cannot be extrapolated to the female population or to other age groups because only 17 young male adults participated in this study. To generalize the findings, further studies based on larger and more heterogeneous cohorts are required.
Identification of differences in physiological states between natural and artificial environments is a critical issue in physiological anthropology, given the increasing disconnect between our evolutionary history and the modern living environment.

Conclusions
The physiological and psychological responses elicited by this field experiment provide evidence for the physiological and psychological benefits of urban green space. A brief spring-time walk in an urban park shifted sympathetic/parasympathetic balance and improved mood state.