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Table 1 Tested genes

From: An adaptive variant of TRIB2, rs1057001, is associated with higher expression levels of thermogenic genes in human subcutaneous and visceral adipose tissues

Symbol Gene name Function Assay type and nucleotide sequences of primersa
CIDEA Cell death-inducing DFFA-like effector A Development of brown/beige adipocytes 5′-CAGCTCGCCCTTTCCGGGTC
5′-CGAGGGCATCCAGAGTCTTGCT
DIO2 Iodothyronine deiodinase 2 Thermogenesis 5′-AGAGGGACTGCGCTGCGTCT
5′-TGCACCACACTGGAATTGGGGG
PDRM16 PR/SET domain 16 Development of brown/beige adipocytes 5′-GAACCAGGCATATGCAATGATGCTG
5′-CCAGCCCGTCAGAGGTGGTTG
PPARGC1A PPARG coactivator 1 alpha Development of brown/beige adipocytes 5′-AGACACCGCACGCACCGAAAT
5′-AGCTGTCATACCTGGGCCGACG
UCP1 Uncoupling protein 1 Thermogenesis 5′-CGGCCTCTACGACACGGTCCA
5′-ACGACCTCTGTGGGTTGCCCAA
ACC Acetyl-CoA carboxylase Fatty acid synthesis TaqMan
DGAT1 Diacylglycerol O-acyltransferase 1 Triglyceride synthesis 5′-GCCCCCAACAAGGACGGAGAC
5′-CCACACACCAGTTCAGGATGCCA
FASN Fatty acid synthase Fatty acid synthesis TaqMan
SCD1 Stearoyl-CoA desaturase 1 Fatty acid synthesis TaqMan
SREBP1 Sterol regulatory element-binding protein1 Master regulator of fatty acid synthesis TaqMan
ADIPOQ Adiponectin Adipocytokine TaqMan
FABP4 Fatty acid-binding protein 4 Incorporation of fatty acids 5′-TGGGGGTGTCCTGGTACATGTGC
5′-ACGCCTTTCATGACGCATTCCACC
ADRB3 Adrenoceptor beta 3 Lipolysis TaqMan
GLUT4 Facilitated glucose transporter, member 4 Incorporation of glucose 5′-TTGGCCCTGGCCCCATTCCT
5′-GCCCCATAGCCTCCGCAACA
LEP Leptin Adipocytokine 5′-TAGGAATCGCAGCGCCAGCGG
5′-ATCCGCACAGGGTTCCCCAATG
  1. aPrimer sequences are shown for genes quantified by RT-PCR with SYBR Green