Table 1 Tested genes
Symbol | Gene name | Function | Assay type and nucleotide sequences of primersa |
|---|---|---|---|
CIDEA | Cell death-inducing DFFA-like effector A | Development of brown/beige adipocytes | 5′-CAGCTCGCCCTTTCCGGGTC 5′-CGAGGGCATCCAGAGTCTTGCT |
DIO2 | Iodothyronine deiodinase 2 | Thermogenesis | 5′-AGAGGGACTGCGCTGCGTCT 5′-TGCACCACACTGGAATTGGGGG |
PDRM16 | PR/SET domain 16 | Development of brown/beige adipocytes | 5′-GAACCAGGCATATGCAATGATGCTG 5′-CCAGCCCGTCAGAGGTGGTTG |
PPARGC1A | PPARG coactivator 1 alpha | Development of brown/beige adipocytes | 5′-AGACACCGCACGCACCGAAAT 5′-AGCTGTCATACCTGGGCCGACG |
UCP1 | Uncoupling protein 1 | Thermogenesis | 5′-CGGCCTCTACGACACGGTCCA 5′-ACGACCTCTGTGGGTTGCCCAA |
ACC | Acetyl-CoA carboxylase | Fatty acid synthesis | TaqMan |
DGAT1 | Diacylglycerol O-acyltransferase 1 | Triglyceride synthesis | 5′-GCCCCCAACAAGGACGGAGAC 5′-CCACACACCAGTTCAGGATGCCA |
FASN | Fatty acid synthase | Fatty acid synthesis | TaqMan |
SCD1 | Stearoyl-CoA desaturase 1 | Fatty acid synthesis | TaqMan |
SREBP1 | Sterol regulatory element-binding protein1 | Master regulator of fatty acid synthesis | TaqMan |
ADIPOQ | Adiponectin | Adipocytokine | TaqMan |
FABP4 | Fatty acid-binding protein 4 | Incorporation of fatty acids | 5′-TGGGGGTGTCCTGGTACATGTGC 5′-ACGCCTTTCATGACGCATTCCACC |
ADRB3 | Adrenoceptor beta 3 | Lipolysis | TaqMan |
GLUT4 | Facilitated glucose transporter, member 4 | Incorporation of glucose | 5′-TTGGCCCTGGCCCCATTCCT 5′-GCCCCATAGCCTCCGCAACA |
LEP | Leptin | Adipocytokine | 5′-TAGGAATCGCAGCGCCAGCGG 5′-ATCCGCACAGGGTTCCCCAATG |