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Table 2 Dilutional linearity of the in-house TPO-Ab ELISA for dried blood spots

From: Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots

DBS sample   Dilution factor
Undiluted 2 4 8
#1 ng/mL 130.5 75.5 29.9 13.8
% Expected value 100.0 115.7 91.7 84.6
#2 ng/mL 49.5 23.0 9.7 5.6
% Expected value 100.0 93.1 78.8 90.7
#3 ng/mL 60.2 26.0 11.6 6.0
% Expected value 100.0 86.3 77.0 80.3
Average % recovery   98.4 82.5 85.2