Table 2 Dilutional linearity of the in-house TPO-Ab ELISA for dried blood spots
DBS sample | Dilution factor | ||||
|---|---|---|---|---|---|
Undiluted | 2 | 4 | 8 | ||
#1 | ng/mL | 130.5 | 75.5 | 29.9 | 13.8 |
% Expected value | 100.0 | 115.7 | 91.7 | 84.6 | |
#2 | ng/mL | 49.5 | 23.0 | 9.7 | 5.6 |
% Expected value | 100.0 | 93.1 | 78.8 | 90.7 | |
#3 | ng/mL | 60.2 | 26.0 | 11.6 | 6.0 |
% Expected value | 100.0 | 86.3 | 77.0 | 80.3 | |
Average % recovery | 98.4 | 82.5 | 85.2 | ||